These results indicated that MPS had a weak interaction with collagen that enhanced its surface negative charge and hydrophobicity without adversely affecting dentin bonding. The MPS10 + MDP and MPS15 + MDP groups presented more silver nitrate and μTBS decrease than the MDP group (P < 0.05). MPS conditioning did not change μTBS and nanoleakage after 24 h of water storage or aging. Water storage for 12 months decreased μTBS (P < 0.05) and increased nanoleakage for all groups. MPS enhanced the hydrophobicity and negative charge of the collagen surface (P 0.05) and reduced fluorescence after 24 h of water storage. According to chemoanalytic characterization and molecular dynamics, a weak interaction between MPS and collagen was observed. In situ zymography was used to evaluate the enzyme activity at the bonded interface. Microtensile bond strength (μTBS) and nanoleakage were evaluated after 24 h or 12 months of water storage. The potential interaction between MPS and collagen was assessed by molecular dynamics, contact angle measurement, zeta potential measurement, and chemoanalytic characterization using X-ray photoelectron spectroscopy, Raman spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, and ultraviolet-visible spectroscopy. Eight groups of primers were prepared: control group, MDP, MPS5, MPS5 + MDP, MPS10, MPS10 + MDP, MPS15, and MPS15 + MDP. In this study, we aimed to examine the effect of 3-methacryloxypropyltrimethoxysilane (MPS) on dentin collagen and the impact of MPS and 10-methacryloyloxydecyl dihydrogen phosphate (MDP) together and separately on resin-dentin bonding. This discovery provides a basis for developing biotechnology for environmental remediation, global warming reduction, and biofertilizer production using Bombyx mori silk and its wastes. Bombyx mori silk also enhance other microbial reactions, including Fe(III)OOH reduction, CO2 reduction to acetate, and nitrogen fixation. The EET function is not degraded by boiling or acid/alkaline treatments and the material can be utilized multiple times, although it is susceptible to UV irradiation. The analyses also suggested the involvement of sulfur-containing amino acids in this function. Subsequently, by dividing fibroin fiber into different soluble/insoluble fractions and correlating their EET functions with their structural properties based on various spectroscopic analyses, the β-sheet configuration is suggested as an essential structure supporting the EET function of silk materials.
In this study, Bombyx mori silk is found, for the first time, to function as an EETM by using an EETM-dependent pentachlorophenol (PCP) dechlorinating anaerobic microbial culture. It is motivated to find out an EETM which is natural-based, environmentally friendly, and easily produced at large-scale. Especially, EETM is known to be essential to activate the energy network in non-electroactive bacteria. Extracellular electron transfer material (EETM) has increasingly attracted attentions for the enhancing effect on multiple microbial reactions.
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